| Pick | Overall | Team | Player | Position | School | State |
| 1 | 572 | Pittsburgh Pirates | Tayler Nunez | RHP | Salmen HS, Slidell, La. | La. |
| 2 | 573 | Seattle Mariners | Luke Guarnaccia | C | Palm Beach (Fla.) CC | Fla. |
| 3 | 574 | Arizona Diamondbacks | Daniel Pulfer | 2B | Oregon | Ore. |
| 4 | 575 | Baltimore Orioles | Dustin Ward | LHP | Central Arkansas | Ark. |
| 5 | 576 | Kansas City Royals | Matt Flemer | RHP | California | Calif. |
| 6 | 577 | Washington Nationals | Hawtin Buchanan | RHP | Biloxi (Miss.) HS | Miss. |
| 7 | 578 | Cleveland Indians | Shawn Morimando | LHP | Ocean Lakes HS, Virginia Beach | Va. |
| 8 | 579 | Chicago Cubs | Paul Hoilman | 1B | East Tennessee State | Tenn. |
| 9 | 580 | Houston Astros | Mitchell Lambson | LHP | Arizona State | Ariz. |
| 10 | 581 | Milwaukee Brewers | Renaldo Jenkins | SS | Whitewater HS, Fayetteville, Ga. | Ga. |
| 11 | 582 | New York Mets | Dustin Lawley | OF | West Florida | Fla. |
| 12 | 583 | Florida Marlins | Connor Burke | 2B | La Serna HS, Whittier, Calif. | Calif. |
| 13 | 584 | Los Angeles Dodgers | Garrett Bush | RHP | Seminole State (Fla.) JC | Fla. |
| 14 | 585 | Los Angeles Angels | Ryan Crowley | LHP | Northwest Florida State JC | Fla. |
| 15 | 586 | Oakland Athletics | Eric Potter | LHP | Maryland | Md. |
| 16 | 587 | Detroit Tigers | Daniel Bennett | RHP | Florida State | Fla. |
| 17 | 588 | Colorado Rockies | Jesse Meaux | RHP | UC Santa Barbara | Calif. |
| 18 | 589 | Toronto Blue Jays | Luke Weaver | RHP | DeLand (Fla.) HS | Fla. |
| 19 | 590 | St. Louis Cardinals | Nick Gillung | LHP | Mercyhurst (Pa.) | Pa. |
| 20 | 591 | Chicago White Sox | Kevin Vance | RHP | Connecticut | Conn. |
| 21 | 592 | Boston Red Sox | Sikes Orvis | 1B | Freedom HS, Orlando | Fla. |
| 22 | 593 | San Diego Padres | Jeremy Gigliotti | LHP | East Stroudsburg (Pa.) | Pa. |
| 23 | 594 | Texas Rangers | Nathan Harsh | LHP | Brunswick (Ga.) HS | Ga. |
| 24 | 595 | Cincinnati Reds | Chris Joyce | LHP | Santa Barbara (Calif.) CC | Calif. |
| 25 | 596 | Atlanta Braves | Troy Snitker | C | North Georgia College & State | Ga. |
| 26 | 597 | San Francisco Giants | Cody Hall | RHP | Southern | La. |
| 27 | 598 | Minnesota Twins | Tyler Koelling | OF | Southern Mississippi | Miss. |
| 28 | 599 | New York Yankees | Robert Paullus | RHP | Memphis | Tenn. |
| 29 | 600 | Tampa Bay Rays | Matt Ramsey | RHP | Tennessee | Tenn. |
| 30 | 601 | Philadelphia Phillies | John Hill | C | Concordia (Calif.) | Calif. |