| Pick | Overall | Team | Player | Position | School | State |
| 1 | 536 | Washington Nationals | Justin Miller | 2B | Middle Tennessee State | Tenn. |
| 2 | 537 | Pittsburgh Pirates | Chase Wentz | OF | Louisiana State-Shreveport | La. |
| 3 | 538 | Baltimore Orioles | Sebastian Vader | RHP | San Marcos (Calif.) HS | Calif. |
| 4 | 539 | Kansas City Royals | Brian Fletcher | OF | Auburn | Ala. |
| 5 | 540 | Cleveland Indians | Chase Burnette | 1B | Georgia Tech | Ga. |
| 6 | 541 | Arizona Diamondbacks | Jimmy Comerota | 1B | Rice | Texas |
| 7 | 542 | New York Mets | A.J. Pinera | RHP | Tampa | Fla. |
| 8 | 543 | Houston Astros | Josh Magee | OF | Hoover (Ala.) HS | Ala. |
| 9 | 544 | San Diego Padres | Dan Meeley | OF | Connors State (Okla.) JC | Okla. |
| 10 | 545 | Oakland Athletics | Jose Macias | RHP | Franklin Pierce (N.H.) | N.H. |
| 11 | 546 | Toronto Blue Jays | Kris Bryant | 3B | Bonanza HS, Las Vegas | Nev. |
| 12 | 547 | Cincinnati Reds | Robert Maddox | OF | Ohio | Ohio |
| 13 | 548 | Chicago White Sox | Randall Thorpe | OF | San Jacinto (Texas) JC | Texas |
| 14 | 549 | Milwaukee Brewers | Thomas Keeling | LHP | Oklahoma State | Okla. |
| 15 | 550 | Chicago Cubs | Brooks Pinckard | OF/RHP | Baylor | Texas |
| 16 | 551 | Tampa Bay Rays | Jimmy Patterson | LHP | Arizona State | Ariz. |
| 17 | 552 | Seattle Mariners | Willy Kesler | RHP | New Mexico | N.M. |
| 18 | 553 | Detroit Tigers | Josh Ashenbrenner | 2B | Lewis-Clark State (Idaho) | Idaho |
| 19 | 554 | Atlanta Braves | Zach Alvord | 2B | South Forsyth HS, Cumming, Ga. | Ga. |
| 20 | 555 | Minnesota Twins | David Gutierrez | RHP | Miami | Fla. |
| 21 | 556 | Texas Rangers | Garrett Buechele | 3B | Oklahoma | Okla. |
| 22 | 557 | Florida Marlins | Corey Goudeau | RHP | Frank Phillips (Texas) JC | Texas |
| 23 | 558 | San Francisco Giants | Brandon Allen | RHP | Milton (Fla.) HS | Fla. |
| 24 | 559 | St. Louis Cardinals | Boone Whiting | RHP | Centenary | La. |
| 25 | 560 | Colorado Rockies | Juan Perez | RHP | Bethune-Cookman | Fla. |
| 26 | 561 | Philadelphia Phillies | Jeff Cusick | 1B | UC Irvine | Calif. |
| 27 | 562 | Los Angeles Dodgers | Chad Arnold | RHP | Washington State | Wash. |
| 28 | 563 | Boston Red Sox | Dallas Chadwick | RHP | Shasta HS, Redding, Calif. | Calif. |
| 29 | 564 | Los Angeles Angels | Ryan Broussard | SS | Louisiana State-Eunice JC | La. |
| 30 | 565 | New York Yankees | Kevin Jacob | RHP | Georgia Tech | Ga. |