| Pick | Overall | Team | Player | Position | School | State |
| 1 | 562 | Washington Nationals | Frank Corolla | RHP | Houston | Texas |
| 2 | 563 | Seattle Mariners | Eric Thomas | RHP | Bethune-Cookman | Fla. |
| 3 | 564 | San Diego Padres | Chris Tremblay | SS | Kent State | Ohio |
| 4 | 565 | Pittsburgh Pirates | Josh Urban | RHP | Dripping Springs (Texas) HS | Texas |
| 5 | 566 | Baltimore Orioles | Kipp Schutz | OF | Indiana | Ind. |
| 6 | 567 | San Francisco Giants | Jason Walls | RHP | Troy | Ala. |
| 7 | 568 | Atlanta Braves | Ty'Relle Harris | RHP | Tennessee | Tenn. |
| 8 | 569 | Cincinnati Reds | Mitch Clarke | LHP | Forest Heights Collegiate Institute, Kitchener, Ont. | Ontario |
| 9 | 570 | Detroit Tigers | Rawley Bishop | 3B | Middle Tennessee State | Tenn. |
| 10 | 571 | Colorado Rockies | Dustin Garneau | C | Cal State Fullerton | Calif. |
| 11 | 572 | Kansas City Royals | Ryan Stovall | 3B | Thomas (Ga.) | Ga. |
| 12 | 573 | Oakland Athletics | Daniel Tenholder | RHP | Austin Peay | Tenn. |
| 13 | 574 | Texas Rangers | Jayce Boyd | 3B | Tate HS, Cantonment, Fla. | Fla. |
| 14 | 575 | Cleveland Indians | Nick Kirk | LHP | Northern Iowa | Iowa |
| 15 | 576 | Arizona Diamondbacks | Randy Hamrick | RHP/SS | Brewton-Parker (Ga.) | Ga. |
| 16 | 577 | Los Angeles Dodgers | Nick Akins | OF | Vanguard (Calif.) | Calif. |
| 17 | 578 | Florida Marlins | Erick Carrillo | RHP | Cal State San Bernardino | Calif. |
| 18 | 579 | St. Louis Cardinals | Travis Tartamella | C | Cal State Los Angeles | Calif. |
| 19 | 580 | Toronto Blue Jays | Ryan Tepera | RHP | Sam Houston State | Texas |
| 20 | 581 | Houston Astros | Brian Kemp | OF | St. John's | N.Y. |
| 21 | 582 | Minnesota Twins | John Stilson | RHP | Texarkana (Texas) CC | Texas |
| 22 | 583 | Chicago White Sox | Brady Shoemaker | OF | Indiana State | Ind. |
| 23 | 584 | New York Mets | Nelfi Zapata | C | English HS, Jamaica Plain, Mass. | Mass. |
| 24 | 585 | New York Yankees | Luke Murton | 1B | Georgia Tech | Ga. |
| 25 | 586 | Milwaukee Brewers | Scott Krieger | OF | George Mason | Va. |
| 26 | 587 | Philadelphia Phillies | Stephen Batts | 1B | East Carolina | N.C. |
| 27 | 588 | Boston Red Sox | Tom Ebert | RHP | Florida International | Fla. |
| 28 | 589 | Tampa Bay Rays | Scott Shuman | RHP | Auburn | Ala. |
| 29 | 590 | Chicago Cubs | Sergio Burruel | C | Browne HS, Phoenix | Ariz. |
| 30 | 591 | Los Angeles Angels | Adam Hornung | OF | Baylor | Texas |